CABRI:ICLC |
Accession_number |
ICLC ATL99008
|
Cell_line_name |
B104
|
Brief_description |
Species: rat, BDIX; Tissue: brain; Tumor: neuroblastoma
|
Description |
Species: rat, BDIX adult; Tissue: brain; Tumor: neuroblastoma
|
Depositor |
Dr F. Tosetti, Molecular Biology Dept IST at ABC, Genoa, Italy
|
Bibliographic_reference |
Nature 1974;249:224-227 [PMID: 4151463
] |
Morphology |
fibroblast and neuroblast, small cells, grown as monolayer
|
Culture_conditions |
continuous culture; DMEM (4,5 g/L) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate; split confluent cultures 1:10-1:20 using trypsin/EDTA (2-3 min.); seed at 1-3 x 104 cells/cm2; 5% CO
|
Viruses |
Search for viruses was not performed
|
Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
|
Hazard |
-
|
Species_validation |
Validated by isoenzymes: confirmed as rat with MD, NP
|
Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
|
Sterility |
mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
|
Further_bibliography |
Brain Res 1981;254:563-575 [PMID: 6458347
] J Neurochem 1999;72:2286-2293 [PMID: 10349837
] Proc Natl Acad Sci USA 1979;76:514-517 [PMID: 284369
]
|